— High-Precision Genome Analysis and High-Efficiency Genome Editing Powered by WGI’s AI, DX, and Big Data Analytics Platform —
◆ In the following descriptions, the letter following each “Task” (e.g., Task A) corresponds to the codes (A–Q) listed under “Outsourcing task(s)” in the Contact Us form.
WGI’s Genome Information Analysis Platform
WGI provides contract analysis services including genome assembly, genome annotation, SNP detection, domain (motif) detection, phylogenetic analysis, and gRNA design for genome editing.
WGI has established a high-speed analysis platform capable of analyzing even large genomes in a short time and possesses extensive experience and a proven track record.
Please specify your desired analysis in the remarks column of the Contact Us form.
Various Genome Analyses Including Genome Assembly, Annotation, SNP Detection, and Domain Analysis (Task J)
◆Genome Information Analysis
WGI offers high-precision analysis services for genome assembly, annotation, SNP detection, DNA marker construction, domain (motif) detection, phylogenetic analysis, and other genome analyses using its proprietary technologies.
We conduct various analyses using publicly available genome data.
Analysis of proprietary NGS datasets is also supported.
GWAS often assumes simple genetic models and requires validation of identified candidate genes.
PAM sites (genomic DNA sequences recognized by PAM, including both genic and intergenic regions) are widely distributed throughout the genome.
For example, it is common to find multiple PAM sites within a single target gene.
The editing efficiency and off-target risk across the entire genome are greatly influenced by the gRNA sequence designed based on the target sequence adjacent to the PAM site.
For example, if mutations are to be introduced in exonic regions, it is necessary to select PAM sites adjacent to target regions that do not include introns.
When aiming to suppress a specific biological function (e.g., enzymatic activity), phenotypic changes may not appear if multiple genes encode the same enzyme and only one gene is knocked out.
Therefore, when creating knockout mutants, it is crucial to identify multiple target genes with the same function,
identify PAM sites enabling simultaneous editing of these genes, and
design gRNAs that do not affect other unrelated genes.
Many existing gRNA design tools assume that the target genes have already been identified in advance.
However, in fields such as breeding, it is common that the genes associated with the trait of interest remain unknown.
By utilizing WGI’s proprietary LA2K technology, it is possible to accurately identify relevant gene sets from trait-associated keywords and
design gRNAs for these gene sets.
Most existing gRNA design tools do not offer the functionality to design gRNAs considering multiple target genes simultaneously.
At WGI, by integrating genome-wide gene function knowledge and comprehensive analysis of PAM sites and gRNA sequences,
we can design optimal gRNAs capable of multiplex editing of multiple target gene sets with
minimal off-target effects.
