— High-Precision Genome Insights and Efficient Editing Driven by WGI’s AI/DX Analytics —
◆ In the following descriptions, the letter following each “Task” (e.g., Task A) corresponds to the codes (A–Q) listed under “Outsourcing task(s)” in the Contact Us form.
WGI’s Genome Information Analysis Platform
WGI provides contract analysis services such as genome assembly, genome annotation, SNP detection, protein domain/motif detection, phylogenetic analysis, and gRNA design for genome editing.
WGI has established a high-speed analysis platform capable of analyzing even large genomes in a short time and possesses extensive experience and a proven track record.
Please specify your desired analysis in the remarks column of the Contact Us form.
Various Genome Analyses Including Genome Assembly, Annotation, SNP Detection, and Domain Analysis (Task J)
◆Genome Information Analysis
WGI offers high-precision analysis services for genome assembly, annotation, SNP detection, DNA marker construction, protein domain and motif detection, phylogenetic analysis, and other comprehensive genome analyses using its proprietary technologies.
We also perform analyses using publicly available genome data.
Analysis of proprietary NGS datasets is also supported.
GWAS often assumes simple genetic models and requires validation of identified candidate genes.
PAM sites (short genomic DNA sequences required for Cas recognition, found in both genic and intergenic regions) are widely distributed throughout the genome.
For example, it is common to find multiple PAM sites within a single target gene.
Genome-wide editing efficiency and off-target risk are strongly affected by the gRNA sequence designed adjacent to the PAM site.
Specifically, if mutations are to be introduced in exonic regions, it is necessary to select PAM sites adjacent to target regions that do not include introns.
If multiple genes encode the same enzyme, knocking out a single gene may not result in observable phenotypic changes when attempting to suppress a specific biological function (e.g., enzymatic activity).
Therefore, when creating knockout mutants, it is crucial to (i) identify multiple target genes with the same function, (ii) locate PAM sites that enable their simultaneous editing, and (iii) design gRNAs that avoid off-target effects on unrelated genes.
Many existing gRNA design tools assume that target genes have already been identified.
However, in fields such as breeding, it is common that the genes associated with the trait of interest remain unknown.
By utilizing WGI’s proprietary LA2K technology, it is possible to accurately identify relevant gene sets from trait-associated keywords and
design gRNAs for these gene sets.
Most existing gRNA design tools do not offer the functionality to design gRNAs considering multiple target genes simultaneously.
At WGI, by integrating genome-wide gene function knowledge with comprehensive analyses of PAM sites and gRNA sequences, we design optimal gRNAs for multiplex editing of target gene sets while minimizing off-target effects.
